Primers Available
Organism:Cucumis sativus mitochondrion chromosome 2
Repeat:(T)10
Start:76250 End:76259
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCGAAAAGGCTTCGTCAATA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GACCGGGTAAAGGAGACGTT | |||||||||||||
| Tm_F_1 | 60.202 | Size_F_1 | 20 | Tm_R_1 | 60.361 | Size_R_1 | 20 | PRODUCT Size | 274 bp | Primer Start | 76079 | Primer End | 76352 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGATACTCGGTTCACATCCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TAAGAAGATCCATGGCCCAC | |||||||||||||
| Tm_F_2 | 59.522 | Size_F_2 | 20 | Tm_R_2 | 59.894 | Size_R_2 | 20 | PRODUCT Size | 266 bp | Primer Start | 76112 | Primer End | 76377 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTATGAGTTTGTCCTGGGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAATAGGGCCGAACTGGTTA | |||||||||||||
| Tm_F_3 | 59.41 | Size_F_3 | 20 | Tm_R_3 | 59.953 | Size_R_3 | 20 | PRODUCT Size | 266 bp | Primer Start | 76157 | Primer End | 76422 |