Primers Available
Organism:Cucumis sativus mitochondrion chromosome 2
Repeat:(GAA)5
Start:58896 End:58910
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGTCTCTGTGGCAGAAGTCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGTCCTCCGGACTATCCATT | |||||||||||||
| Tm_F_1 | 60.178 | Size_F_1 | 20 | Tm_R_1 | 60.154 | Size_R_1 | 20 | PRODUCT Size | 263 bp | Primer Start | 58683 | Primer End | 58945 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCTCCTAAAACTCTCCGGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTCCTCCGGACTATCCATT | |||||||||||||
| Tm_F_2 | 59.571 | Size_F_2 | 20 | Tm_R_2 | 60.154 | Size_R_2 | 20 | PRODUCT Size | 130 bp | Primer Start | 58816 | Primer End | 58945 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCTCCTAAAACTCTCCGGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCAATATTCTTTGCGCCTTC | |||||||||||||
| Tm_F_3 | 59.571 | Size_F_3 | 20 | Tm_R_3 | 59.822 | Size_R_3 | 20 | PRODUCT Size | 175 bp | Primer Start | 58816 | Primer End | 58990 |