Primers Available
Organism:Pyrus pyrifolia chloroplast
Repeat:(T)24
Start:66621 End:66644
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGAATTTTACCCAACCCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTGTCCACTACCACTATGTTACG | |||||||||||||
| Tm_F_1 | 60.048 | Size_F_1 | 20 | Tm_R_1 | 58.2 | Size_R_1 | 24 | PRODUCT Size | 173 bp | Primer Start | 66536 | Primer End | 66708 |
| FORWARD_PRIMER_2 | |||||||||||||
| AACGGAATTTTACCCAACCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTGTCCACTACCACTATGTTACG | |||||||||||||
| Tm_F_2 | 59.924 | Size_F_2 | 20 | Tm_R_2 | 58.2 | Size_R_2 | 24 | PRODUCT Size | 175 bp | Primer Start | 66534 | Primer End | 66708 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACGGAATTTTACCCAACCCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTGTCCACTACCACTATGTTACG | |||||||||||||
| Tm_F_3 | 59.571 | Size_F_3 | 20 | Tm_R_3 | 58.2 | Size_R_3 | 24 | PRODUCT Size | 174 bp | Primer Start | 66535 | Primer End | 66708 |