Primers Available
Organism:Pyrus pyrifolia chloroplast
Repeat:(T)19acttat(TTTA)3
Start:83025 End:83061
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACTCCCTTGGGGATTCTACG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGAAATCTAAAGGACCCGA | |||||||||||||
| Tm_F_1 | 60.319 | Size_F_1 | 20 | Tm_R_1 | 59.64 | Size_R_1 | 20 | PRODUCT Size | 232 bp | Primer Start | 82865 | Primer End | 83096 |
| FORWARD_PRIMER_2 | |||||||||||||
| CATGAATAACTCCCTTGGGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGAAATCTAAAGGACCCGA | |||||||||||||
| Tm_F_2 | 59.24 | Size_F_2 | 20 | Tm_R_2 | 59.64 | Size_R_2 | 20 | PRODUCT Size | 240 bp | Primer Start | 82857 | Primer End | 83096 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTACGCGAACCAATTTTTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATGTCGATTGATCCGTAGGC | |||||||||||||
| Tm_F_3 | 58.816 | Size_F_3 | 20 | Tm_R_3 | 59.923 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 82915 | Primer End | 83194 |