Primers Available
Organism:Pyrus pyrifolia chloroplast
Repeat:(T)10
Start:27355 End:27364
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCCGCCTACACAAGCAAAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGCTCGTGTGAGTAGGAAA | |||||||||||||
| Tm_F_1 | 60.27 | Size_F_1 | 20 | Tm_R_1 | 60.255 | Size_R_1 | 20 | PRODUCT Size | 201 bp | Primer Start | 27294 | Primer End | 27494 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACTACCGGATCTCCGCCTAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGATATGGGCTCGTGTGAGT | |||||||||||||
| Tm_F_2 | 60.484 | Size_F_2 | 20 | Tm_R_2 | 59.957 | Size_R_2 | 20 | PRODUCT Size | 217 bp | Primer Start | 27284 | Primer End | 27500 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACTACCGGATCTCCGCCTAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGATATGGGCTCGTGTGAG | |||||||||||||
| Tm_F_3 | 60.484 | Size_F_3 | 20 | Tm_R_3 | 60.096 | Size_R_3 | 20 | PRODUCT Size | 218 bp | Primer Start | 27284 | Primer End | 27501 |