Primers Available
Organism:Pyrus pyrifolia chloroplast
Repeat:(AATTG)3aatcaaaataaaattccaatccaaactcaaatgcggattgacg(T)11
Start:71722 End:71790
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAGTCAACCGCTAGAGCTGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGAGAATGAACTCTGGGAAGG | |||||||||||||
| Tm_F_1 | 60.307 | Size_F_1 | 20 | Tm_R_1 | 60.059 | Size_R_1 | 21 | PRODUCT Size | 280 bp | Primer Start | 71665 | Primer End | 71944 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAGTCAACCGCTAGAGCTGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGACAGGACATTTCGATTTT | |||||||||||||
| Tm_F_2 | 60.307 | Size_F_2 | 20 | Tm_R_2 | 57.67 | Size_R_2 | 20 | PRODUCT Size | 159 bp | Primer Start | 71665 | Primer End | 71823 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAGTCAACCGCTAGAGCTGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACGACAGGACATTTCGATTTT | |||||||||||||
| Tm_F_3 | 60.307 | Size_F_3 | 20 | Tm_R_3 | 58.567 | Size_R_3 | 21 | PRODUCT Size | 160 bp | Primer Start | 71665 | Primer End | 71824 |