Primers Available
Organism:Pyrus pyrifolia chloroplast
Repeat:(A)22ttttatcttaattaattgtttctgagtcaccggttcttatttcttttcttttgaaaggggtcggttaat(A)10
Start:116439 End:116539
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGTTGCCGTTGGAAAAAGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGAAAGATTCAGAATAATTAAGGC | |||||||||||||
| Tm_F_1 | 60.514 | Size_F_1 | 20 | Tm_R_1 | 59.132 | Size_R_1 | 25 | PRODUCT Size | 264 bp | Primer Start | 116338 | Primer End | 116601 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGTTGCCGTTGGAAAAAGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGAAAGATTCAGAATAATTAAGGCT | |||||||||||||
| Tm_F_2 | 60.514 | Size_F_2 | 20 | Tm_R_2 | 59.912 | Size_R_2 | 26 | PRODUCT Size | 264 bp | Primer Start | 116338 | Primer End | 116601 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGTTGCCGTTGGAAAAAGTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGAAAGATTCAGAATAATTAAGGC | |||||||||||||
| Tm_F_3 | 59.202 | Size_F_3 | 20 | Tm_R_3 | 59.132 | Size_R_3 | 25 | PRODUCT Size | 263 bp | Primer Start | 116339 | Primer End | 116601 |