Primers Available
Organism:Pyrus pyrifolia chloroplast
Repeat:(A)13gaatcaatgtgtagatgtagattctagcgttttct(TTTA)3(T)15cataccaggcttttaacttataaaaaggggcttttctttcatttttcaaaaaagatgggttttggcctgttttgtttatctat(A)11
Start:74642 End:74810
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCACCTTTTGATGCATACG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CATCGCGATTGAGATTGTATC | |||||||||||||
| Tm_F_1 | 60.665 | Size_F_1 | 20 | Tm_R_1 | 58.201 | Size_R_1 | 21 | PRODUCT Size | 275 bp | Primer Start | 74604 | Primer End | 74878 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCACCTTTTGATGCATACG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACATCGCGATTGAGATTGTATC | |||||||||||||
| Tm_F_2 | 60.665 | Size_F_2 | 20 | Tm_R_2 | 59.074 | Size_R_2 | 22 | PRODUCT Size | 276 bp | Primer Start | 74604 | Primer End | 74879 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGATGCATACGGTTCAACAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CATCGCGATTGAGATTGTATC | |||||||||||||
| Tm_F_3 | 59.984 | Size_F_3 | 21 | Tm_R_3 | 58.201 | Size_R_3 | 21 | PRODUCT Size | 266 bp | Primer Start | 74613 | Primer End | 74878 |