Primers Available
Organism:Pyrus pyrifolia chloroplast
Repeat:(A)10gtgcattagaagtgtcgttcg(A)16
Start:68686 End:68732
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGGGTCCTTCGTGACCTAAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGAATCACTCTGGAATAAAATTGG | |||||||||||||
| Tm_F_1 | 60.19 | Size_F_1 | 20 | Tm_R_1 | 59.395 | Size_R_1 | 24 | PRODUCT Size | 269 bp | Primer Start | 68532 | Primer End | 68800 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCGATTGATCTTATCGTGCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAATCACTCTGGAATAAAATTGG | |||||||||||||
| Tm_F_2 | 59.792 | Size_F_2 | 20 | Tm_R_2 | 59.395 | Size_R_2 | 24 | PRODUCT Size | 199 bp | Primer Start | 68602 | Primer End | 68800 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGGTCCTTCGTGACCTAAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCACTCTGGAATAAAATTGGAAAA | |||||||||||||
| Tm_F_3 | 60.19 | Size_F_3 | 20 | Tm_R_3 | 59.067 | Size_R_3 | 24 | PRODUCT Size | 265 bp | Primer Start | 68532 | Primer End | 68796 |