Primers Available
Organism:Hucho bleekeri mitochondrion
Repeat:(T)13
Start:539 End:551
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCTTAAGAAACCAGCCCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGAGGATGGAATTGGACCTT | |||||||||||||
| Tm_F_1 | 60.068 | Size_F_1 | 20 | Tm_R_1 | 59.336 | Size_R_1 | 20 | PRODUCT Size | 279 bp | Primer Start | 426 | Primer End | 704 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTTATTGCCCGTTACCCAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAGGATGGAATTGGACCTT | |||||||||||||
| Tm_F_2 | 59.823 | Size_F_2 | 20 | Tm_R_2 | 59.336 | Size_R_2 | 20 | PRODUCT Size | 222 bp | Primer Start | 483 | Primer End | 704 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTTATTGCCCGTTACCCAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGTGAGGATGGAATTGGACC | |||||||||||||
| Tm_F_3 | 59.823 | Size_F_3 | 20 | Tm_R_3 | 60.725 | Size_R_3 | 20 | PRODUCT Size | 224 bp | Primer Start | 483 | Primer End | 706 |