Primers Available
Organism:Marssonina brunnea f. sp. 'multigermtubi' mitochondrion
Repeat:(A)12
Start:27461 End:27472
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGGGAATTAGGTAGCCTGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATATCGCATATGTTTGGCCT | |||||||||||||
| Tm_F_1 | 60.278 | Size_F_1 | 20 | Tm_R_1 | 57.1 | Size_R_1 | 20 | PRODUCT Size | 268 bp | Primer Start | 27382 | Primer End | 27649 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGTAGCCTGGATTAAATGCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATATCGCATATGTTTGGCCT | |||||||||||||
| Tm_F_2 | 59.569 | Size_F_2 | 20 | Tm_R_2 | 57.1 | Size_R_2 | 20 | PRODUCT Size | 259 bp | Primer Start | 27391 | Primer End | 27649 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGTGGGGAATTAGGTAGCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATATCGCATATGTTTGGCCT | |||||||||||||
| Tm_F_3 | 59.307 | Size_F_3 | 20 | Tm_R_3 | 57.1 | Size_R_3 | 20 | PRODUCT Size | 272 bp | Primer Start | 27378 | Primer End | 27649 |