Primers Available
Organism:Ichthyophthirius multifiliis mitochondrion
Repeat:(TAA)4
Start:623 End:634
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATAAATGATTCGGGGTTGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTGATTGGTAAAAGCGGCAG | |||||||||||||
| Tm_F_1 | 60.744 | Size_F_1 | 20 | Tm_R_1 | 60.762 | Size_R_1 | 20 | PRODUCT Size | 279 bp | Primer Start | 534 | Primer End | 812 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGTTGGGCCCTGTATATTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTGGCTGATTGGTAAAAGC | |||||||||||||
| Tm_F_2 | 58.647 | Size_F_2 | 20 | Tm_R_2 | 59.574 | Size_R_2 | 20 | PRODUCT Size | 272 bp | Primer Start | 546 | Primer End | 817 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGTTGGGCCCTGTATATTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGTGGCTGATTGGTAAAAG | |||||||||||||
| Tm_F_3 | 58.647 | Size_F_3 | 20 | Tm_R_3 | 59.429 | Size_R_3 | 20 | PRODUCT Size | 273 bp | Primer Start | 546 | Primer End | 818 |