Primers Available
Organism:Ichthyophthirius multifiliis mitochondrion
Repeat:(TAA)4
Start:398 End:409
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGGTTGGGCCCTGTATATTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCAACCCCGAATCATTTAT | |||||||||||||
| Tm_F_1 | 58.647 | Size_F_1 | 20 | Tm_R_1 | 60.744 | Size_R_1 | 20 | PRODUCT Size | 233 bp | Primer Start | 321 | Primer End | 553 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGTTGGGCCCTGTATATTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCAACCCCGAATCATTTATTT | |||||||||||||
| Tm_F_2 | 58.647 | Size_F_2 | 20 | Tm_R_2 | 59.902 | Size_R_2 | 21 | PRODUCT Size | 232 bp | Primer Start | 321 | Primer End | 552 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGTTGGGCCCTGTATATTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCCAACCCCGAATCATTTA | |||||||||||||
| Tm_F_3 | 58.647 | Size_F_3 | 20 | Tm_R_3 | 60.508 | Size_R_3 | 19 | PRODUCT Size | 233 bp | Primer Start | 321 | Primer End | 553 |