Primers Available
Organism:Ichthyophthirius multifiliis mitochondrion
Repeat:(T)11
Start:48252 End:48262
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACGGTTTCAATTGGGATTCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TACAACCTGCCGCTTTTACC | |||||||||||||
| Tm_F_1 | 60.17 | Size_F_1 | 20 | Tm_R_1 | 60.131 | Size_R_1 | 20 | PRODUCT Size | 135 bp | Primer Start | 48167 | Primer End | 48301 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGAACGGTTTCAATTGGGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TACAACCTGCCGCTTTTACC | |||||||||||||
| Tm_F_2 | 60.17 | Size_F_2 | 20 | Tm_R_2 | 60.131 | Size_R_2 | 20 | PRODUCT Size | 138 bp | Primer Start | 48164 | Primer End | 48301 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTTGAACGGTTTCAATTGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TACAACCTGCCGCTTTTACC | |||||||||||||
| Tm_F_3 | 60.336 | Size_F_3 | 20 | Tm_R_3 | 60.131 | Size_R_3 | 20 | PRODUCT Size | 140 bp | Primer Start | 48162 | Primer End | 48301 |