Primers Available
Organism:Ichthyophthirius multifiliis mitochondrion
Repeat:(ATAA)3
Start:10838 End:10849
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGTGGTATTCCAGAAAATGAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCATATTTGGAAAATACTTCTATTCA | |||||||||||||
| Tm_F_1 | 58.303 | Size_F_1 | 22 | Tm_R_1 | 58.573 | Size_R_1 | 27 | PRODUCT Size | 254 bp | Primer Start | 10695 | Primer End | 10948 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTAAAAATGGTGGTATTCCAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCCATATTTGGAAAATACTTCTATTCA | |||||||||||||
| Tm_F_2 | 59.277 | Size_F_2 | 23 | Tm_R_2 | 58.573 | Size_R_2 | 27 | PRODUCT Size | 262 bp | Primer Start | 10687 | Primer End | 10948 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCAGAAAAACCTAAAAATGGTGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCATATTTGGAAAATACTTCTATTCA | |||||||||||||
| Tm_F_3 | 60.898 | Size_F_3 | 23 | Tm_R_3 | 58.573 | Size_R_3 | 27 | PRODUCT Size | 271 bp | Primer Start | 10678 | Primer End | 10948 |