Primers Available
Organism:Ichthyophthirius multifiliis mitochondrion
Repeat:(AT)8a(AAAAT)3
Start:12261 End:12292
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTTTTGGTGAAATTGATCGTTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTTAATTCGTGTCTACCTAAACTGAT | |||||||||||||
| Tm_F_1 | 57.265 | Size_F_1 | 22 | Tm_R_1 | 57.232 | Size_R_1 | 27 | PRODUCT Size | 280 bp | Primer Start | 12191 | Primer End | 12470 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTGGTGAAATTGATCGTTAATAAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCGTGTCTACCTAAACTGATAAATCT | |||||||||||||
| Tm_F_2 | 59.186 | Size_F_2 | 26 | Tm_R_2 | 58.114 | Size_R_2 | 27 | PRODUCT Size | 272 bp | Primer Start | 12193 | Primer End | 12464 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGGTGAAATTGATCGTTAATAAAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCGTGTCTACCTAAACTGATAAATCT | |||||||||||||
| Tm_F_3 | 58.057 | Size_F_3 | 25 | Tm_R_3 | 58.114 | Size_R_3 | 27 | PRODUCT Size | 271 bp | Primer Start | 12194 | Primer End | 12464 |