Primers Available
Organism:Ichthyophthirius multifiliis mitochondrion
Repeat:(AT)6
Start:33702 End:33713
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAACTAAATAGCGTCTAGGTGAGTAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCACTGCTGTCAAAAGATAGAGTT | |||||||||||||
| Tm_F_1 | 57.628 | Size_F_1 | 27 | Tm_R_1 | 59.025 | Size_R_1 | 24 | PRODUCT Size | 280 bp | Primer Start | 33642 | Primer End | 33921 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCATTACACATGCAAGTTAAATTAGGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGTTGAACAATCTTAAAATTCAACT | |||||||||||||
| Tm_F_2 | 60.827 | Size_F_2 | 27 | Tm_R_2 | 59.42 | Size_R_2 | 26 | PRODUCT Size | 280 bp | Primer Start | 33601 | Primer End | 33880 |
| FORWARD_PRIMER_3 | |||||||||||||
| CATTACACATGCAAGTTAAATTAGGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGTTGAACAATCTTAAAATTCAACT | |||||||||||||
| Tm_F_3 | 57.863 | Size_F_3 | 26 | Tm_R_3 | 59.42 | Size_R_3 | 26 | PRODUCT Size | 279 bp | Primer Start | 33602 | Primer End | 33880 |