Primers Available
Organism:Ichthyophthirius multifiliis mitochondrion
Repeat:(AT)6
Start:19078 End:19089
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATTGAATACCTTGTTGAAATAAAAAGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCAATTTGAGCCTTACAAGAGTT | |||||||||||||
| Tm_F_1 | 57.172 | Size_F_1 | 27 | Tm_R_1 | 58.572 | Size_R_1 | 24 | PRODUCT Size | 198 bp | Primer Start | 18967 | Primer End | 19164 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTGAATACCTTGTTGAAATAAAAAGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTTCAATTTGAGCCTTACAAGAGTT | |||||||||||||
| Tm_F_2 | 57.172 | Size_F_2 | 27 | Tm_R_2 | 59.312 | Size_R_2 | 25 | PRODUCT Size | 199 bp | Primer Start | 18967 | Primer End | 19165 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTGAATACCTTGTTGAAATAAAAAGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTTCAATTTGAGCCTTACAAGAG | |||||||||||||
| Tm_F_3 | 57.172 | Size_F_3 | 27 | Tm_R_3 | 57.285 | Size_R_3 | 23 | PRODUCT Size | 199 bp | Primer Start | 18967 | Primer End | 19165 |