Primers Available
Organism:Ichthyophthirius multifiliis mitochondrion
Repeat:(AAT)5
Start:11849 End:11863
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGTCAACATGGTGATTCATACG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGAACTTCAGTTTTTGAAGTGATT | |||||||||||||
| Tm_F_1 | 60.118 | Size_F_1 | 22 | Tm_R_1 | 57.189 | Size_R_1 | 24 | PRODUCT Size | 252 bp | Primer Start | 11731 | Primer End | 11982 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGTCAACATGGTGATTCATACG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAACTTCAGTTTTTGAAGTGATTA | |||||||||||||
| Tm_F_2 | 60.118 | Size_F_2 | 22 | Tm_R_2 | 57.293 | Size_R_2 | 25 | PRODUCT Size | 252 bp | Primer Start | 11731 | Primer End | 11982 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGTCAACATGGTGATTCATACG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGAAGTGATTAATTAAAGATTCCATTG | |||||||||||||
| Tm_F_3 | 60.118 | Size_F_3 | 22 | Tm_R_3 | 58.988 | Size_R_3 | 27 | PRODUCT Size | 238 bp | Primer Start | 11731 | Primer End | 11968 |