Primers Available
Organism:Ichthyophthirius multifiliis mitochondrion
Repeat:(A)12
Start:33471 End:33482
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGTGCCCTACCATTTCAACA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGTTAATTCCGAGCCATGAT | |||||||||||||
| Tm_F_1 | 59.964 | Size_F_1 | 20 | Tm_R_1 | 59.923 | Size_R_1 | 20 | PRODUCT Size | 193 bp | Primer Start | 33398 | Primer End | 33590 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGTGCCCTACCATTTCAACA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATCAGCGTTAATTCCGAGC | |||||||||||||
| Tm_F_2 | 59.964 | Size_F_2 | 20 | Tm_R_2 | 59.324 | Size_R_2 | 20 | PRODUCT Size | 199 bp | Primer Start | 33398 | Primer End | 33596 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGTGCCCTACCATTTCAACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGAGCCATGATCACACTCAA | |||||||||||||
| Tm_F_3 | 59.964 | Size_F_3 | 20 | Tm_R_3 | 60.84 | Size_R_3 | 20 | PRODUCT Size | 184 bp | Primer Start | 33398 | Primer End | 33581 |