Primers Available
Organism:Ichthyophthirius multifiliis mitochondrion
Repeat:(A)10
Start:830 End:839
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TACAACCTGCCGCTTTTACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACGGTTTCAATTGGGATTCA | |||||||||||||
| Tm_F_1 | 60.131 | Size_F_1 | 20 | Tm_R_1 | 60.17 | Size_R_1 | 20 | PRODUCT Size | 136 bp | Primer Start | 787 | Primer End | 922 |
| FORWARD_PRIMER_2 | |||||||||||||
| TACAACCTGCCGCTTTTACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAACGGTTTCAATTGGGAT | |||||||||||||
| Tm_F_2 | 60.131 | Size_F_2 | 20 | Tm_R_2 | 60.17 | Size_R_2 | 20 | PRODUCT Size | 139 bp | Primer Start | 787 | Primer End | 925 |
| FORWARD_PRIMER_3 | |||||||||||||
| TACAACCTGCCGCTTTTACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTTGAACGGTTTCAATTGGG | |||||||||||||
| Tm_F_3 | 60.131 | Size_F_3 | 20 | Tm_R_3 | 60.336 | Size_R_3 | 20 | PRODUCT Size | 141 bp | Primer Start | 787 | Primer End | 927 |