Primers Available
Organism:Ichthyophthirius multifiliis mitochondrion
Repeat:(A)10
Start:27850 End:27859
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCTTTCAATAAAATCAAATAAGGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAACTTTCTCCGATTTATGGTCA | |||||||||||||
| Tm_F_1 | 59.045 | Size_F_1 | 25 | Tm_R_1 | 59.048 | Size_R_1 | 23 | PRODUCT Size | 274 bp | Primer Start | 27797 | Primer End | 28070 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCTTTCAATAAAATCAAATAAGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGAGGTTGAGAATTTTATGGTGA | |||||||||||||
| Tm_F_2 | 59.045 | Size_F_2 | 25 | Tm_R_2 | 59.878 | Size_R_2 | 24 | PRODUCT Size | 233 bp | Primer Start | 27797 | Primer End | 28029 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCTTTCAATAAAATCAAATAAGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AACTTTCTCCGATTTATGGTCA | |||||||||||||
| Tm_F_3 | 59.045 | Size_F_3 | 25 | Tm_R_3 | 57.748 | Size_R_3 | 22 | PRODUCT Size | 273 bp | Primer Start | 27797 | Primer End | 28069 |