Primers Available
Organism:Chaetomium thermophilum var. thermophilum DSM 1495 mitochondrion
Repeat:(ATGT)3
Start:15032 End:15043
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTCTAGTGCGACATCCGTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAGGATACATTCCCCTCCCC | |||||||||||||
| Tm_F_1 | 60.008 | Size_F_1 | 20 | Tm_R_1 | 59.974 | Size_R_1 | 20 | PRODUCT Size | 279 bp | Primer Start | 14847 | Primer End | 15125 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGCTCCGTAAGGATGGGACT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AACGCCTCAAATGTACCGAC | |||||||||||||
| Tm_F_2 | 60.096 | Size_F_2 | 20 | Tm_R_2 | 60 | Size_R_2 | 20 | PRODUCT Size | 150 bp | Primer Start | 15006 | Primer End | 15155 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGCTCCGTAAGGATGGGACT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACTGAGACGACGTGAGGCTT | |||||||||||||
| Tm_F_3 | 60.096 | Size_F_3 | 20 | Tm_R_3 | 60.057 | Size_R_3 | 20 | PRODUCT Size | 211 bp | Primer Start | 15006 | Primer End | 15216 |