Primers Available
Organism:Diploid Xenocypris davidi x Megalobrama amblycephala mitochondrion
Repeat:(TTA)4
Start:11719 End:11730
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACCATGTCAATCCTGAGCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCGGTGGATTGTTGGAGTAG | |||||||||||||
| Tm_F_1 | 59.934 | Size_F_1 | 20 | Tm_R_1 | 60.366 | Size_R_1 | 20 | PRODUCT Size | 272 bp | Primer Start | 11573 | Primer End | 11844 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAGCCTATTAGCCGACAACG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCGGTGGATTGTTGGAGTAG | |||||||||||||
| Tm_F_2 | 59.867 | Size_F_2 | 20 | Tm_R_2 | 60.366 | Size_R_2 | 20 | PRODUCT Size | 257 bp | Primer Start | 11588 | Primer End | 11844 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAGCCTATTAGCCGACAACG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTTGAGGCGCTCAGTTTGAT | |||||||||||||
| Tm_F_3 | 59.867 | Size_F_3 | 20 | Tm_R_3 | 60.406 | Size_R_3 | 20 | PRODUCT Size | 174 bp | Primer Start | 11588 | Primer End | 11761 |