Primers Available
Organism:Diploid Xenocypris davidi x Megalobrama amblycephala mitochondrion
Repeat:(TA)8
Start:812 End:827
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCTTGTCAAACCCCAAAAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGTGAATTAATGCGATGTG | |||||||||||||
| Tm_F_1 | 59.807 | Size_F_1 | 20 | Tm_R_1 | 59.953 | Size_R_1 | 20 | PRODUCT Size | 119 bp | Primer Start | 732 | Primer End | 850 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAACCCCAAAACCAAGGAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGGTGAATTAATGCGATGTG | |||||||||||||
| Tm_F_2 | 60.193 | Size_F_2 | 20 | Tm_R_2 | 59.953 | Size_R_2 | 20 | PRODUCT Size | 111 bp | Primer Start | 740 | Primer End | 850 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAACCCCAAAACCAAGGAAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTTGGAGAATTTTGCGGTG | |||||||||||||
| Tm_F_3 | 60.193 | Size_F_3 | 20 | Tm_R_3 | 59.938 | Size_R_3 | 20 | PRODUCT Size | 126 bp | Primer Start | 740 | Primer End | 865 |