Primers Available
Organism:Silurus asotus mitochondrion
Repeat:(CTCC)3ttc(CTA)4
Start:5780 End:5806
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TACCAATTATGATCGGGGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AATGGCCCCAAGAATAGAGG | |||||||||||||
| Tm_F_1 | 59.972 | Size_F_1 | 20 | Tm_R_1 | 60.278 | Size_R_1 | 20 | PRODUCT Size | 275 bp | Primer Start | 5678 | Primer End | 5952 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTTGGAAACTGGCTTGTGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAAATTAATGGCCCCAAGA | |||||||||||||
| Tm_F_2 | 60.118 | Size_F_2 | 20 | Tm_R_2 | 60.26 | Size_R_2 | 20 | PRODUCT Size | 263 bp | Primer Start | 5697 | Primer End | 5959 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTCCCCCGAATGAATAACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGAAATTAATGGCCCCAAGA | |||||||||||||
| Tm_F_3 | 60.124 | Size_F_3 | 20 | Tm_R_3 | 60.26 | Size_R_3 | 20 | PRODUCT Size | 215 bp | Primer Start | 5745 | Primer End | 5959 |