Primers Available
Organism:Pimelodus pictus mitochondrion
Repeat:(AACC)3
Start:8473 End:8484
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTGGCAGTACCCCTGTGACT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGTTTGCTGTCAATCGGAC | |||||||||||||
| Tm_F_1 | 60.174 | Size_F_1 | 20 | Tm_R_1 | 60.119 | Size_R_1 | 20 | PRODUCT Size | 182 bp | Primer Start | 8427 | Primer End | 8608 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTGGCAGTACCCCTGTGACT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGAATTAGGGGAGTAGGGG | |||||||||||||
| Tm_F_2 | 60.174 | Size_F_2 | 20 | Tm_R_2 | 60.138 | Size_R_2 | 20 | PRODUCT Size | 108 bp | Primer Start | 8427 | Primer End | 8534 |
| FORWARD_PRIMER_3 | |||||||||||||
| CACAACAACTGCTAACCCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGAGTAGGGGTTCCTTCTGG | |||||||||||||
| Tm_F_3 | 59.609 | Size_F_3 | 20 | Tm_R_3 | 59.928 | Size_R_3 | 20 | PRODUCT Size | 247 bp | Primer Start | 8278 | Primer End | 8524 |