Primers Available
Organism:Nerodia sipedon mitochondrion
Repeat:(TCCT)3
Start:3877 End:3888
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCATGACTATCCCGTTCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TATAAAGGGTGCGACCAAGG | |||||||||||||
| Tm_F_1 | 60.195 | Size_F_1 | 20 | Tm_R_1 | 59.953 | Size_R_1 | 20 | PRODUCT Size | 227 bp | Primer Start | 3815 | Primer End | 4041 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTAGCCCAGCCTGTGAAATC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGCAAGTAGGGAGGGATGGT | |||||||||||||
| Tm_F_2 | 60.214 | Size_F_2 | 20 | Tm_R_2 | 59.957 | Size_R_2 | 20 | PRODUCT Size | 160 bp | Primer Start | 3851 | Primer End | 4010 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTAGCCCAGCCTGTGAAATC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TATAAAGGGTGCGACCAAGG | |||||||||||||
| Tm_F_3 | 60.214 | Size_F_3 | 20 | Tm_R_3 | 59.953 | Size_R_3 | 20 | PRODUCT Size | 191 bp | Primer Start | 3851 | Primer End | 4041 |