Primers Available
Organism:Clarias sp. NM-2010 mitochondrion
Repeat:(AGC)5
Start:4223 End:4237
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCAATCCTCCCCCTAATAGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGTTTGGTCTGTTAGGCAT | |||||||||||||
| Tm_F_1 | 59.894 | Size_F_1 | 20 | Tm_R_1 | 59.993 | Size_R_1 | 20 | PRODUCT Size | 166 bp | Primer Start | 4148 | Primer End | 4313 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCAATCCTCCCCCTAATAGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGGGGCTAGGCCTACTTTT | |||||||||||||
| Tm_F_2 | 59.894 | Size_F_2 | 20 | Tm_R_2 | 60.089 | Size_R_2 | 20 | PRODUCT Size | 217 bp | Primer Start | 4148 | Primer End | 4364 |
| FORWARD_PRIMER_3 | |||||||||||||
| CACGAGCAGTAGAGGCAACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGGTTTGGTCTGTTAGGCAT | |||||||||||||
| Tm_F_3 | 60.199 | Size_F_3 | 20 | Tm_R_3 | 59.993 | Size_R_3 | 20 | PRODUCT Size | 132 bp | Primer Start | 4182 | Primer End | 4313 |