Primers Available
Organism:Polycyathus sp. MFL-2011 mitochondrion
Repeat:(A)10
Start:92 End:101
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GACTAAGGGTCAGTCTTCGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCCGCCATTTCGAAAAATA | |||||||||||||
| Tm_F_1 | 60.117 | Size_F_1 | 21 | Tm_R_1 | 60.385 | Size_R_1 | 20 | PRODUCT Size | 146 bp | Primer Start | 9 | Primer End | 154 |
| FORWARD_PRIMER_2 | |||||||||||||
| TAAGGGTCAGTCTTCGGGCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCCGCCATTTCGAAAAATA | |||||||||||||
| Tm_F_2 | 61.142 | Size_F_2 | 20 | Tm_R_2 | 60.385 | Size_R_2 | 20 | PRODUCT Size | 143 bp | Primer Start | 12 | Primer End | 154 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTAAGGGTCAGTCTTCGGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCCGCCATTTCGAAAAATA | |||||||||||||
| Tm_F_3 | 61.142 | Size_F_3 | 20 | Tm_R_3 | 60.385 | Size_R_3 | 20 | PRODUCT Size | 144 bp | Primer Start | 11 | Primer End | 154 |