Primers Available
Organism:Pseudobagrus brevicorpus mitochondrion
Repeat:(CTCC)3
Start:5783 End:5794
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATGGCATTCCCACGAATAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGGTCTGGTATTGGGAGAT | |||||||||||||
| Tm_F_1 | 60.153 | Size_F_1 | 20 | Tm_R_1 | 60.015 | Size_R_1 | 20 | PRODUCT Size | 272 bp | Primer Start | 5743 | Primer End | 6014 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGATATGGCATTCCCACGAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGGTCTGGTATTGGGAGAT | |||||||||||||
| Tm_F_2 | 60.296 | Size_F_2 | 20 | Tm_R_2 | 60.015 | Size_R_2 | 20 | PRODUCT Size | 276 bp | Primer Start | 5739 | Primer End | 6014 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATGGCATTCCCACGAATAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATTGCCCCTAGAATGGAGG | |||||||||||||
| Tm_F_3 | 60.153 | Size_F_3 | 20 | Tm_R_3 | 60.278 | Size_R_3 | 20 | PRODUCT Size | 213 bp | Primer Start | 5743 | Primer End | 5955 |