Primers Available
Organism:Pipa carvalhoi mitochondrion
Repeat:(CCT)4
Start:4374 End:4385
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACGCCTGAAACTCAGGAGAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGGTAGAAGTGAGCCCGAT | |||||||||||||
| Tm_F_1 | 59.989 | Size_F_1 | 20 | Tm_R_1 | 60.096 | Size_R_1 | 20 | PRODUCT Size | 259 bp | Primer Start | 4183 | Primer End | 4441 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTAGCCCCATTCCACTTCTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CACCCTAAGTGGGCGATAGA | |||||||||||||
| Tm_F_2 | 60.066 | Size_F_2 | 20 | Tm_R_2 | 60.088 | Size_R_2 | 20 | PRODUCT Size | 245 bp | Primer Start | 4275 | Primer End | 4519 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTAGCCCCATTCCACTTCTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGGTAGAAGTGAGCCCGAT | |||||||||||||
| Tm_F_3 | 60.066 | Size_F_3 | 20 | Tm_R_3 | 60.096 | Size_R_3 | 20 | PRODUCT Size | 167 bp | Primer Start | 4275 | Primer End | 4441 |