Primers Available
Organism:Nelumbo lutea chloroplast
Repeat:(TAAAAA)3gaactttcg(A)14tacttatgtgaataaaagtaaaatactact(AAATA)3(AATAA)3
Start:201 End:301
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACATCCGCCCCTCTACTCTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGTTGAAGTTGAAGGAACAA | |||||||||||||
| Tm_F_1 | 60.617 | Size_F_1 | 20 | Tm_R_1 | 58.392 | Size_R_1 | 21 | PRODUCT Size | 280 bp | Primer Start | 83 | Primer End | 362 |
| FORWARD_PRIMER_2 | |||||||||||||
| CATCCGCCCCTCTACTCTCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGTTGAAGTTGAAGGAACAA | |||||||||||||
| Tm_F_2 | 60.746 | Size_F_2 | 20 | Tm_R_2 | 58.392 | Size_R_2 | 21 | PRODUCT Size | 279 bp | Primer Start | 84 | Primer End | 362 |
| FORWARD_PRIMER_3 | |||||||||||||
| CATCCGCCCCTCTACTCTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTCGTTGAAGTTGAAGGAACAA | |||||||||||||
| Tm_F_3 | 60.746 | Size_F_3 | 20 | Tm_R_3 | 59.262 | Size_R_3 | 22 | PRODUCT Size | 280 bp | Primer Start | 84 | Primer End | 363 |