Primers Available
Organism:Nicholsicypris normalis mitochondrion
Repeat:(TTA)4
Start:10774 End:10785
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TAATCCTGAGCCAATCAGCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCTGCATTAAGGCGTTCAGT | |||||||||||||
| Tm_F_1 | 60.177 | Size_F_1 | 20 | Tm_R_1 | 60.27 | Size_R_1 | 20 | PRODUCT Size | 186 bp | Primer Start | 10636 | Primer End | 10821 |
| FORWARD_PRIMER_2 | |||||||||||||
| TAATCCTGAGCCAATCAGCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTTCCTGCATTAAGGCGTTC | |||||||||||||
| Tm_F_2 | 60.177 | Size_F_2 | 20 | Tm_R_2 | 59.713 | Size_R_2 | 20 | PRODUCT Size | 189 bp | Primer Start | 10636 | Primer End | 10824 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATCAGCCGACAACGACTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCTGCATTAAGGCGTTCAGT | |||||||||||||
| Tm_F_3 | 59.74 | Size_F_3 | 20 | Tm_R_3 | 60.27 | Size_R_3 | 20 | PRODUCT Size | 174 bp | Primer Start | 10648 | Primer End | 10821 |