Primers Available
Organism:Nicholsicypris normalis mitochondrion
Repeat:(CTT)4
Start:6075 End:6086
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTCGTCAATCTTAGGGGCAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCTGCAGGGTCAAAGAATGT | |||||||||||||
| Tm_F_1 | 60.074 | Size_F_1 | 20 | Tm_R_1 | 60.111 | Size_R_1 | 20 | PRODUCT Size | 207 bp | Primer Start | 5959 | Primer End | 6165 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCAGCCATTTCCCAGTATC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCTGCAGGGTCAAAGAATGT | |||||||||||||
| Tm_F_2 | 59.894 | Size_F_2 | 20 | Tm_R_2 | 60.111 | Size_R_2 | 20 | PRODUCT Size | 153 bp | Primer Start | 6013 | Primer End | 6165 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTCGTCAATCTTAGGGGCAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TAAGATTGGGTCCCCTCCTC | |||||||||||||
| Tm_F_3 | 60.074 | Size_F_3 | 20 | Tm_R_3 | 60.264 | Size_R_3 | 20 | PRODUCT Size | 226 bp | Primer Start | 5959 | Primer End | 6184 |