Primers Available
Organism:Pichia pastoris CBS 7435 mitochondrion
Repeat:(ATA)4
Start:21407 End:21418
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACGGACTACGTTCGATCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAAAACAACCATCCCCATCT | |||||||||||||
| Tm_F_1 | 59.942 | Size_F_1 | 19 | Tm_R_1 | 59.647 | Size_R_1 | 20 | PRODUCT Size | 240 bp | Primer Start | 21235 | Primer End | 21474 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGACGGACTACGTTCGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAAAACAACCATCCCCATCT | |||||||||||||
| Tm_F_2 | 59.942 | Size_F_2 | 19 | Tm_R_2 | 59.647 | Size_R_2 | 20 | PRODUCT Size | 243 bp | Primer Start | 21232 | Primer End | 21474 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACGGACTACGTTCGATCCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTCCAAAACAACCATCCCC | |||||||||||||
| Tm_F_3 | 59.942 | Size_F_3 | 19 | Tm_R_3 | 60.787 | Size_R_3 | 20 | PRODUCT Size | 244 bp | Primer Start | 21235 | Primer End | 21478 |