Primers Available
Organism:Pichia pastoris CBS 7435 mitochondrion
Repeat:(AT)6
Start:9530 End:9541
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGGAATTTCAATTTCATGGAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCAATCCATCTTCCCCTGTC | |||||||||||||
| Tm_F_1 | 58.288 | Size_F_1 | 22 | Tm_R_1 | 59.862 | Size_R_1 | 20 | PRODUCT Size | 187 bp | Primer Start | 9408 | Primer End | 9594 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGGAATTTCAATTTCATGGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CATCAATCCATCTTCCCCTG | |||||||||||||
| Tm_F_2 | 58.288 | Size_F_2 | 22 | Tm_R_2 | 60.272 | Size_R_2 | 20 | PRODUCT Size | 189 bp | Primer Start | 9408 | Primer End | 9596 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGGAATTTCAATTTCATGGAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCATCAATCCATCTTCCCCT | |||||||||||||
| Tm_F_3 | 58.288 | Size_F_3 | 22 | Tm_R_3 | 59.291 | Size_R_3 | 20 | PRODUCT Size | 190 bp | Primer Start | 9408 | Primer End | 9597 |