Primers Available
Organism:Pichia pastoris CBS 7435 mitochondrion
Repeat:(AT)12
Start:31214 End:31237
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CATTCAATTTCTGGGGTCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTGGTCTCTTTATAAATGCTTAATTT | |||||||||||||
| Tm_F_1 | 57.3 | Size_F_1 | 20 | Tm_R_1 | 57.428 | Size_R_1 | 27 | PRODUCT Size | 280 bp | Primer Start | 31093 | Primer End | 31372 |
| FORWARD_PRIMER_2 | |||||||||||||
| CATTCAATTTCTGGGGTCATAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTGGTCTCTTTATAAATGCTTAATTT | |||||||||||||
| Tm_F_2 | 58.807 | Size_F_2 | 22 | Tm_R_2 | 57.428 | Size_R_2 | 27 | PRODUCT Size | 280 bp | Primer Start | 31093 | Primer End | 31372 |
| FORWARD_PRIMER_3 | |||||||||||||
| CATTCAATTTCTGGGGTCATA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTGGTCTCTTTATAAATGCTTAATTT | |||||||||||||
| Tm_F_3 | 57.415 | Size_F_3 | 21 | Tm_R_3 | 57.428 | Size_R_3 | 27 | PRODUCT Size | 280 bp | Primer Start | 31093 | Primer End | 31372 |