Primers Available
Organism:Pichia pastoris CBS 7435 mitochondrion
Repeat:(AAAAAG)3
Start:429 End:446
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CATAGTCCAATTGATGGGGAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCATGCATTGTTAAACCTAATGT | |||||||||||||
| Tm_F_1 | 59.631 | Size_F_1 | 21 | Tm_R_1 | 58.02 | Size_R_1 | 23 | PRODUCT Size | 244 bp | Primer Start | 331 | Primer End | 574 |
| FORWARD_PRIMER_2 | |||||||||||||
| CATAGTCCAATTGATGGGGAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGATGTAGTTAATGGTCAAGGTGAA | |||||||||||||
| Tm_F_2 | 59.631 | Size_F_2 | 21 | Tm_R_2 | 59.821 | Size_R_2 | 25 | PRODUCT Size | 197 bp | Primer Start | 331 | Primer End | 527 |
| FORWARD_PRIMER_3 | |||||||||||||
| CATAGTCCAATTGATGGGGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCATGCATTGTTAAACCTAATGT | |||||||||||||
| Tm_F_3 | 59.631 | Size_F_3 | 21 | Tm_R_3 | 58.803 | Size_R_3 | 24 | PRODUCT Size | 245 bp | Primer Start | 331 | Primer End | 575 |