Primers Available
Organism:Phoxinus percnurus mitochondrion
Repeat:(TCC)4
Start:6061 End:6072
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACAGTATACCCGCCACTTGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAAGTATTGTAATCCCGGCG | |||||||||||||
| Tm_F_1 | 60.022 | Size_F_1 | 20 | Tm_R_1 | 59.433 | Size_R_1 | 20 | PRODUCT Size | 250 bp | Primer Start | 5861 | Primer End | 6110 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGTATACCCGCCACTTGCAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAAGTATTGTAATCCCGGCG | |||||||||||||
| Tm_F_2 | 60.154 | Size_F_2 | 20 | Tm_R_2 | 59.433 | Size_R_2 | 20 | PRODUCT Size | 248 bp | Primer Start | 5863 | Primer End | 6110 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCCCAGCTACTTCCCAATA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCTGCTGGGTCAAAGAATGT | |||||||||||||
| Tm_F_3 | 61.18 | Size_F_3 | 20 | Tm_R_3 | 60.111 | Size_R_3 | 20 | PRODUCT Size | 155 bp | Primer Start | 5999 | Primer End | 6153 |