Primers Available
Organism:Chlorella variabilis plastid
Repeat:(T)12aaat(A)10
Start:32851 End:32876
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATAACCGTACACAAGCGCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGCCACCTTATCGTTTTCC | |||||||||||||
| Tm_F_1 | 60.025 | Size_F_1 | 20 | Tm_R_1 | 60.32 | Size_R_1 | 20 | PRODUCT Size | 245 bp | Primer Start | 32674 | Primer End | 32918 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATAACCGTACACAAGCGCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCACCTTATCGTTTTCCGAC | |||||||||||||
| Tm_F_2 | 60.025 | Size_F_2 | 20 | Tm_R_2 | 59.429 | Size_R_2 | 20 | PRODUCT Size | 242 bp | Primer Start | 32674 | Primer End | 32915 |
| FORWARD_PRIMER_3 | |||||||||||||
| CATGGTGTGCCATTTGTTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACAGGCTCACGGATACCATC | |||||||||||||
| Tm_F_3 | 59.297 | Size_F_3 | 20 | Tm_R_3 | 59.957 | Size_R_3 | 20 | PRODUCT Size | 228 bp | Primer Start | 32787 | Primer End | 33014 |