Primers Available
Organism:Chlorella variabilis plastid
Repeat:(AAAG)3
Start:65872 End:65883
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTTTTGAGATGGCAAGGGAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCAAGGAAGACCCATACGAA | |||||||||||||
| Tm_F_1 | 60.184 | Size_F_1 | 20 | Tm_R_1 | 59.926 | Size_R_1 | 20 | PRODUCT Size | 199 bp | Primer Start | 65730 | Primer End | 65928 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACGCATCGTTCCAAAAAGAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CATACCTTGACGCCACATTG | |||||||||||||
| Tm_F_2 | 60.249 | Size_F_2 | 20 | Tm_R_2 | 59.988 | Size_R_2 | 20 | PRODUCT Size | 280 bp | Primer Start | 65814 | Primer End | 66093 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGCATCGTTCCAAAAAGAGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CATACCTTGACGCCACATTG | |||||||||||||
| Tm_F_3 | 60.249 | Size_F_3 | 20 | Tm_R_3 | 59.988 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 65815 | Primer End | 66093 |