Primers Available
Organism:Carduelis sinica mitochondrion
Repeat:(ACCA)3
Start:15374 End:15385
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGCCCAAGACTCAGGATAAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAGGCCGAGCAGGAATTTAT | |||||||||||||
| Tm_F_1 | 59.83 | Size_F_1 | 20 | Tm_R_1 | 60.061 | Size_R_1 | 20 | PRODUCT Size | 267 bp | Primer Start | 15255 | Primer End | 15521 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCGAATAAACGAACACCACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGGCCGAGCAGGAATTTAT | |||||||||||||
| Tm_F_2 | 60.227 | Size_F_2 | 20 | Tm_R_2 | 60.061 | Size_R_2 | 20 | PRODUCT Size | 229 bp | Primer Start | 15293 | Primer End | 15521 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGAATAAACGAACACCACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCGTCTAATCCGTCTCCTT | |||||||||||||
| Tm_F_3 | 60.227 | Size_F_3 | 20 | Tm_R_3 | 59.836 | Size_R_3 | 20 | PRODUCT Size | 155 bp | Primer Start | 15293 | Primer End | 15447 |