Primers Available
Organism:Brachyhypopomus occidentalis mitochondrion
Repeat:(T)11
Start:16187 End:16197
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACTCGACATCTCCATGACCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCGGAGGGGGAATCTTATAG | |||||||||||||
| Tm_F_1 | 59.928 | Size_F_1 | 20 | Tm_R_1 | 59.888 | Size_R_1 | 20 | PRODUCT Size | 250 bp | Primer Start | 16133 | Primer End | 16382 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACTCGACATCTCCATGACCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAAACGTAGTTTTGGCGGA | |||||||||||||
| Tm_F_2 | 59.928 | Size_F_2 | 20 | Tm_R_2 | 59.625 | Size_R_2 | 20 | PRODUCT Size | 265 bp | Primer Start | 16133 | Primer End | 16397 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGACTCGACATCTCCATGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCGGAGGGGGAATCTTATAG | |||||||||||||
| Tm_F_3 | 60.363 | Size_F_3 | 20 | Tm_R_3 | 59.888 | Size_R_3 | 20 | PRODUCT Size | 253 bp | Primer Start | 16130 | Primer End | 16382 |