Primers Available
Organism:Brachyhypopomus occidentalis mitochondrion
Repeat:(CCTT)3
Start:5742 End:5753
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGGCAACTGACTTATCCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAGACCCCTGCAAGATGAAG | |||||||||||||
| Tm_F_1 | 59.933 | Size_F_1 | 20 | Tm_R_1 | 59.803 | Size_R_1 | 20 | PRODUCT Size | 235 bp | Primer Start | 5655 | Primer End | 5889 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGACATAGCATTCCCACGAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGACCCCTGCAAGATGAAG | |||||||||||||
| Tm_F_2 | 60.073 | Size_F_2 | 20 | Tm_R_2 | 59.803 | Size_R_2 | 20 | PRODUCT Size | 198 bp | Primer Start | 5692 | Primer End | 5889 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGACATAGCATTCCCACGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCTGCAAGATGAAGGGAGAA | |||||||||||||
| Tm_F_3 | 60.073 | Size_F_3 | 20 | Tm_R_3 | 60.331 | Size_R_3 | 20 | PRODUCT Size | 192 bp | Primer Start | 5692 | Primer End | 5883 |