Primers Available
Organism:Pungitius sinensis mitochondrion
Repeat:(T)12
Start:16170 End:16181
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCTCCCACTTTTATCGACG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTTGACGCGAAAGATGTTCA | |||||||||||||
| Tm_F_1 | 60.066 | Size_F_1 | 20 | Tm_R_1 | 59.988 | Size_R_1 | 20 | PRODUCT Size | 177 bp | Primer Start | 16075 | Primer End | 16251 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCTGGCATTTGGTTCCTAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTTGACGCGAAAGATGTTCA | |||||||||||||
| Tm_F_2 | 59.933 | Size_F_2 | 20 | Tm_R_2 | 59.988 | Size_R_2 | 20 | PRODUCT Size | 221 bp | Primer Start | 16031 | Primer End | 16251 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGGTTATTGAAGGTGAGGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTTGACGCGAAAGATGTTCA | |||||||||||||
| Tm_F_3 | 59.926 | Size_F_3 | 20 | Tm_R_3 | 59.988 | Size_R_3 | 20 | PRODUCT Size | 275 bp | Primer Start | 15977 | Primer End | 16251 |