Primers Available
Organism:Pungitius sinensis mitochondrion
Repeat:(C)15
Start:7160 End:7174
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CACCTTTGAAGAGCCTGCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCTGCGTCCTGAAATCCTA | |||||||||||||
| Tm_F_1 | 60.397 | Size_F_1 | 20 | Tm_R_1 | 59.978 | Size_R_1 | 20 | PRODUCT Size | 250 bp | Primer Start | 6967 | Primer End | 7216 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCACCACACCTTTGAAGAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGCTGCGTCCTGAAATCCTA | |||||||||||||
| Tm_F_2 | 60.536 | Size_F_2 | 20 | Tm_R_2 | 59.978 | Size_R_2 | 20 | PRODUCT Size | 257 bp | Primer Start | 6960 | Primer End | 7216 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCTTTGAAGAGCCTGCATTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCTGCGTCCTGAAATCCTA | |||||||||||||
| Tm_F_3 | 59.452 | Size_F_3 | 20 | Tm_R_3 | 59.978 | Size_R_3 | 20 | PRODUCT Size | 248 bp | Primer Start | 6969 | Primer End | 7216 |