Primers Available
Organism:Gobiobotia brevibarba mitochondrion
Repeat:(TTA)4
Start:10762 End:10773
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCACCTCTCTTCAGGCCTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAGGTTCCCGCATTTAGTCG | |||||||||||||
| Tm_F_1 | 59.53 | Size_F_1 | 20 | Tm_R_1 | 60.089 | Size_R_1 | 20 | PRODUCT Size | 148 bp | Primer Start | 10668 | Primer End | 10815 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCACCTCTCTTCAGGCCTTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTAGTCGCTCGGTCTGGTTT | |||||||||||||
| Tm_F_2 | 59.53 | Size_F_2 | 20 | Tm_R_2 | 59.875 | Size_R_2 | 20 | PRODUCT Size | 135 bp | Primer Start | 10668 | Primer End | 10802 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCACCTCTCTTCAGGCCTTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTAGTCGCTCGGTCTGGTT | |||||||||||||
| Tm_F_3 | 59.53 | Size_F_3 | 20 | Tm_R_3 | 59.875 | Size_R_3 | 20 | PRODUCT Size | 136 bp | Primer Start | 10668 | Primer End | 10803 |