Primers Available
Organism:Gobiobotia brevibarba mitochondrion
Repeat:(G)14
Start:4548 End:4561
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCCCTGGGGTTGATGTCTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTGGCGGATGATGTTTTTA | |||||||||||||
| Tm_F_1 | 59.92 | Size_F_1 | 20 | Tm_R_1 | 59.93 | Size_R_1 | 20 | PRODUCT Size | 211 bp | Primer Start | 4519 | Primer End | 4729 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCGCTACTTCTAACCTCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTGGCGGATGATGTTTTTA | |||||||||||||
| Tm_F_2 | 60.089 | Size_F_2 | 20 | Tm_R_2 | 59.93 | Size_R_2 | 20 | PRODUCT Size | 226 bp | Primer Start | 4504 | Primer End | 4729 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTTTGAATGCCAGAGGTCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCATTCAGCCTATGTGAGCA | |||||||||||||
| Tm_F_3 | 60.051 | Size_F_3 | 20 | Tm_R_3 | 59.823 | Size_R_3 | 20 | PRODUCT Size | 236 bp | Primer Start | 4390 | Primer End | 4625 |