Primers Available
Organism:Cottus poecilopus mitochondrion
Repeat:(CCCT)3
Start:3611 End:3622
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GATGAACACGCTATCAGCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGCTATAGCTGTGGAGGCAG | |||||||||||||
| Tm_F_1 | 59.83 | Size_F_1 | 20 | Tm_R_1 | 60 | Size_R_1 | 20 | PRODUCT Size | 261 bp | Primer Start | 3564 | Primer End | 3824 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTGCCCCCTTTGACTTAACA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CATGAGCTGGTCGTATCGAA | |||||||||||||
| Tm_F_2 | 59.971 | Size_F_2 | 20 | Tm_R_2 | 59.823 | Size_R_2 | 20 | PRODUCT Size | 275 bp | Primer Start | 3449 | Primer End | 3723 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTGCCCCCTTTGACTTAACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCTGCTTTGGTCATGAGAT | |||||||||||||
| Tm_F_3 | 59.971 | Size_F_3 | 20 | Tm_R_3 | 60.226 | Size_R_3 | 20 | PRODUCT Size | 209 bp | Primer Start | 3449 | Primer End | 3657 |